![]() ![]() The Center for Personalized Cancer Therapy is a collaborative venture between the University of Massachusetts Boston and the Dana Farber/Harvard Cancer Center (DF/HCC) and funded by the Massachusetts Life Sciences Center. Additional instruments include a Biomek FXp automated workstation, and a QuantStudio 12K Flex Real-Time PCR System. and 10x Genomics today announced a collaboration to integrate use of the 10x Genomics Chromium Single Cell Gene Expression solutions with Ultimas advanced. The Core offers cost-effective massively parallel sequencing with the Illumina HiSeq 2500 (v4, 1TB enabled) and Illumina MiSeq platforms 10X Genomics Chromium Single Cell Sequencing Platform Nanostring nCounter Analysis System next-generation sequencing library preparation from RNA and DNA, and nucleic acid quality assessments using Bioanalyzer, Tapestation, Nanodrop and Qubit assays. ![]() The 10x Genomics Chromium platform provides advanced. Microbiology, Boston University School of Medicine, 650 Albany St, Boston. We help investigators and clinicians analyze samples, identify genetic variants contributing to disease risk, and reveal complex mechanisms involved in human disease. Single-cell RNA sequencing analyzes gene expression at single-cell resolution for heterogeneous samples. The Core leverages cutting-edge technologies and next-generation sequencing capabilities for research and clinical applications. Next Generation of Single Cell The Chromium X Series lets you access single cell analysis at any stage. Chromium Single Cell 3’ performs deep profiling of complex cell populations with high-throughput digital gene expression on a cell-by-cell basis. Easy to use Simple upgrades, intuitive interfaces, direct support. The following protocol describes the steps for single cell mRNA-seq, but the platform is capable of other analyses including cell surface protein expression, ATAC-seq, dual RNAseq/ATACseq, and Spatial Transcriptomics on intact tissue sections. ![]() Accessible Enjoy economies of scale with larger-scope experiments. We use the 10X Chromium Controller platform to encapsulate and barcode single cells. The CPCT Genomics Core enables UMass Boston investigators and academic and industry collaborators to carry out basic and translational genomics research. Flexible Low to high throughput, handles all single cell assays. ![]()
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